If you are like me when you first opened up BEAST phylogenetics software, or more specifically BEAUTi GUI, you are immediately impressed but a little overwhelmed by the number of options you have to do reconstruction and phylogenetic analysis more broadly. It’s an amazingly powerful and accessible free software package – particularly with the extra utilities such as Fig Tree and SPREAD (will talk about these in future posts) you really can’t beat BEAST. The tutorials (see below) provide step by step instructions basically mean that anyone can run BEAST. Furthermore, If you have any technical problems, they are often quickly resolved via the google group (https://groups.google.com/forum/#!forum/beast-users).
Despite this, understanding the numerous decisions that have to made throughout the process from sequence alignment to final product is the real challenge here. These decisions can make real differences to the inferences you make so are critical to get right. One weakness in the tutorials is that they tell you how, but don’t give enough detail to why you’d make a particular decision (e.g., why one tree prior over another?). The aims of the following posts will be to demystify this process a little and direct you to useful resources.
The plan is to do this tab by tab of BEAUti and I will assume that you know how to import your data in and set dates/traits (all of this can be learnt from the tutorials easily).
Part 2 – Sites
Part 3 – Clocks
Part 4 – Trees
Part 5 -States, Priors and Operators
Part 6 – Running the whole thing and model selection
In the mean time, if you haven’t already, download BEAST 1.8.4 (http://beast.bio.ed.ac.uk/tutorials) and go through the tutorials: http://beast.bio.ed.ac.uk/tutorials.
The idea that there is reduced disease exposure risk in systems with high biodiversity is certainly an attractive one. Basically more species in an ecosystem ‘dilutes’ the chances of a parasite coming across a good host. The argument follows that protecting biodiversity can have positive implications for human health. This idea however has sparked a massive amount of controversy and passionate debate – see an excellent summary of the debate here: the https://parasiteecology.wordpress.com/2013/12/08/dilution-effect-debates-randolph-and-dobson-vs-ostfeld/
This idea originating from a Conservation Biology paper by Ostfeld and Keesing (see the link below) on Lyme disease has been labelled everything from ‘visionary’ to a ‘fantasy’. I have mixed feeling about the paper – but I think the idea at least is an interesting one worthy of the attention that it gets. How to even conduct research to test this idea properly is contentious- in a meeting a few years back people that have devoted their professional careers on this idea cannot come to a consensus. Adding further complexity, is that there is still debate on how to measure biodiversity effectively in the first place (even though there have been huge advances – See the article by Chiarucci et al below). As with most ecological research, there are also issues working at what scale to asses the effect and what species to use etc.
Unsurprisingly (if you have read my previous articles) I think that a functional and phylogenetic community ecology approach has potential to overcome some of these issues at least. What I feel is needed to answer this question appropriately is a study design with multiple sites across known diversity gradients at multiple scales (any ideas anyone?). This study design coupled with vertebrate surveys (rather than relying on coarse distribution data) and next-gen metagenomic sampling symbionts/parasites from a sample of the hosts. Using functional diversity and composition with taxonomic diversity and composition metrics of the host (and maybe the parasites?) would be useful too – and may allow for more broad applicability of the findings i.e. even though the host species composition is different the functional composition is the same.
Anyway if anyone has any ideas and may like to work on a similar project in the future – let me know!
The original Ostfeld and Keesing paper: http://onlinelibrary.wiley.com/doi/10.1046/j.1523-1739.2000.99014.x/abstract
Chiarucci et al diversity article: http://rstb.royalsocietypublishing.org/content/366/1576/2426